Crosslinking-MS

At the moment, data validation is only available for crosslinking-MS data deposited as a fully compliant dataset in the PRIDE Crosslinking database. Correspondence between crosslinking-MS and entry entities is established using pyHMMER. Only residue pairs that passed the reported threshold are used for the analysis. The values in the report have to be interpreted in the context of the experiment (i.e. only a minor fraction of in-situ or in-vivo dataset can be used for modeling).

Crosslinking-MS dataset is not available in the PRIDE Crosslinking database.

3DEM

This section describes quality of the 3DEM datasets

3DEM dataset (EMDB) EMD-50218
Resolution: 27.30 Å
Recommended level: 0.600
Estimated volume: 775.95 nm³
Map projections ?
Orthogonal projection ?
Only data for the representative model is shown Only data for the representative model is shown
X Y Z
Orthogonal standard-deviation projections (false-colour) ?
Only data for the representative model is shown
X Y Z
Orthogonal surface views (at contour level: 0.600 ?
Only data for the representative model is shown
X Y Z
Map analysis ?
This section contains the results of statistical analysis of the map.
Map-value distribution ?

The map-value distribution is plotted in 128 intervals along the x-axis. The y-axis is logarithmic. A spike in this graph at zero usually indicates that the volume has been masked.

Volume estimate ?
The volume at the recommended contour level is 775.95 nm³. The volume estimate graph shows how the enclosed volume varies with the contour level. The recommended contour level is shown as a vertical line and the intersection between the line and the curve gives the volume of the enclosed surface at the given leve
Rotationally averaged power spectrum ?

*Reported resolution corresponds to spatial frequency of 0.037 Å⁻¹

Fourier-Shell correlation ?
FSC ?
Fourier-Shell Correlation (FSC) is the most commonly used method to estimate the resolution of single-parttiiiie and subtomogram-averaged maps. The shape of the curve depends on the imposed symmetry, mmaaaa and whether or not the two 3D reconstructions used were processed from a common refeerrrrce. The reported resolution is shown as a black line. A curve is displayed for the half-bit crriiiirion in addition to lines showing the 0.143 gold standard cut-off and 0.5 cut-off.

*Reported resolution corresponds to spatial frequency of 0.037 Å⁻¹

Resolution estimates ?